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PROVAXS is embedded in the University of Ghent

PROVAXS is the coordinating center of a network of laboratories of the University of Ghent that unite their expertise in the development of veterinary and human vaccines, therapeutics and diagnostics

PROVAXS focuses on those projects and research results that respond to an urgent need in society or in the market and can lead to industrial applications. We can therefore rely on specific funds for valorization that are available at our University and on the continuous support of Technology Transfer, the central department at the University of Ghent that assists in the protection and licensing of our intellectual property and helps to build strategic alliances.


Mission

PROVAXS wants to facilitate the transfer of new technologies in the domain of veterinary and human vaccines, therapeutics and diagnostics that result from multidisciplinary research to a practical and/or industrial application, thus realising a return to fundamental research.


Vision

PROVAXS is an interface that, with attention for the quality of high-end scientific research, is open for the needs and demands of our industrial partners and offers them solutions and opportunities in a pro-active way.


Benefits & Value

PROVAXS offers a multidisciplinary environment for research and development directed towards technologies with practical applications in industry

PROVAXS offers expertise in mucosal immunology, pathogen-host interactions of viruses and parasites, biomarkers of infections, immune response and immune diseases, related diagnostics, formulations for drugs and vaccines

PROVAXS can be your gateway to participation in a network of high-quality R&D in various research programs at national and international level

Via partnering PROVAXS can offer you access to gap-funding, an extra tool to stimulate and accelerate valorization of new technologies


The PROVAXS consortium of UGent laboratories

PROVAXS joins the forces of laboratories of four different faculties of the University of Ghent

Faculty of Veterinary Medicine :  Department of Virology, Parasitology and Immunology, Laboratory of Bacteriology

Faculty of Medicine and Health Sciences : Centre for Vaccinology, Department of Rheumatology, Centre for Cllinical Immunology and Allergy

Faculty of Bioscience Engineering : Laboratory for Immunology and Animal Biotechnology, Laboratory for Biochemistry and Molecular Cytology

Faculty of Pharmacy : Laboratory of Pharmaceutical Technology, Laboratory of Pharmaceutical Biotechnology, Laboratory of General Biochemistry and Physical Pharmacy


Laboratory of Virology

Research

In the Laboratory of Virology, the research is focused on the animal, cellular and molecular pathogenesis of viruses, with a special emphasis on viruses that have developed strategies to escape from the host's immune response.

Projects are focused on the following topics :

  • Pseudorabies virus
  • Porcine reproductive and respiratory syndrome virus (PRRSV)
  • Influenza
  • Equine herpesvirus Type 1
  • Feline infectious peritonitis virus
  • Porcine circovirus Type 2
  • Porcine respiratory coronavirus
  • White spot syndrome virus
  • Murine cytomegalovirus

Website

www.vetvirology.ugent.be


Laboratory of Parasitology

Research

In the Laboratory of Parasitology, the research is mainly focused on the study of gastro-intestinal parasites and their interactions with the host animal.

Projects are focused on the following topics :
  • The epidemiology and control of cattle nematodes
  • Vaccination against the abomasal cattle nematode Ostertagia ostertagi
  • Macrocyclic lactone resistance in the nematodes Ostertagia ostertagi and Cooperia onchophora.
  • Role of Cryptosporidium parvum and Giardia spp. in neonatal diarrhoea complex in ruminants
  • The epidemiology of cysticercosis and trichinellosis in Vietnam, Zambia and Ecuador
  • The epidemiology of animal schistosomiasis in Africa
  • Miscellaneous topics on the epidemiology, diagnosis and control of parasite infection in horses, swine and exotic animals


Services

We offer analysis of
  • Cryptosporidiosis
  • Lungworms
  • Determination of parasites
  • Coccidiosis
  • Tapeworm
  • Pepsinogen
  • Gastrointestinal nematodes
  • Fasciola
  • Giardia
  • Cyathostominae
  • Ectoparasites

Website

www.vetparasitology.ugent.be

Laboratory of Immunology

Research

Our expertise on mucosal immunity is based on more than 15 years of research in extensive cooperation with several national and international research institutes.

In vivo models (e.g. F4 in pigs) are used to gain insights in the mucosal immune mechanisms as well as to develop vaccination strategies for the induction of protective mucosal immunity.

Examples of applied strategies for mucosal immunizations are :
  • Parenteral DNA vaccination
  • Parenteral protein vaccination
  • Immunisation targeted to epithelial cells or DCs
  • Oral vaccination with antigen in enteric coated pellets
  • Oral vaccination with nanoparticles
Furthermore adjuvants are evaluated in this models such as CpG, GM-CSF, Vitamin D3, ß-glucans, inulin and nucleotides.

Starting from the results obtained in the in vivo models we develop in vitro systems for the evaluation and optimisation of adjuvants and antigens.

New research will be initiated on allergies, immunopathologies that are becoming increasingly important and are related to the field of mucosal immunology.

Another part of the research focuses on the interplay between alphaherpesviruses and the immune system. Alphaherpesviruses establish lifelong infections in neurons of their hosts in a latent, dormant form and can be sporadically reactivated. Our aim is to develop systems to study the mechanisms of virus latency and reactivation cycles and to develop strategies to interfere with these cycles.

Services

A number of diagnostic assays for diagnosing auto-immune diseases and immunodeficiencies are available. Combining different assays enables us to analyze the effect of various factors (oligosaccharides, nucleotides, vitamins, fatty acids and others) on mucosal and systemic vaccination.
  • Flow cytometry : CD2 or CD3, CD4, CD8+ T cells, CD11a+ cells, IgM+ B-cells, monocytes/macrophages, neutrophils
  • IgA, IgG, IgM, IgE concentrations in serum of horses, donkeys, dogs and cattle
  • IgA antibodies in cerebrospinal fluid of dogs
  • Lymphocyte proliferation assays in all animal species
  • Cytokine detection in swine and dogs
  • F18-verotoxigenic E. coli specific serum and milk antibodies by ELISA
  • F4-enterotoxigenic E. coli specific serum and milk antibodies
  • Anti-nuclear antibody concentration in serum of horses and dogs (ANA test)
  • Anti-erythrocyte antibody concentration in horses, dogs and cats (COOMBS test)
  • Canine rheumafactor (Rheuma test)
  • Tissue-specific auto-antibodies using indirect immunofluorescence
  • Canine leukocyte adhesion deficiency by PCR and restriction enzyme digestion
  • Bovine leukocyte adhesion deficiency by PCR and restriction enzyme digestion
  • Receptor for F18+ verotoxigenic E. coli by PCR and restriction enzyme digestion

Diagnosis
  • Immunodeficiencies (at the level of B-lymphocytes, T-lymphocytes, monocytes, neutrophiles, IgM, IgA, IgG) in all species
  • Total IgE concentrations in dogs (allergy)
  • Systemic lupus erythematosus (SLE)
  • Autoimmune hemolytic anemia (AIHA)
  • Corticoid-responsive meningitis
  • BLAD
  • CLAD
Website
www.vetimmunology.ugent.be

 


Centre for Vaccinology

Research

The Center for Vaccinology (CEVAC) conducts research on the improvement of existing and the development of new vaccines. In it’s 20 year history, CEVAC has participated in more than 50 clinical vaccine evaluations. In this way, CEVAC has been involved in the development and evaluation of vaccines against hepatitis B, the first combined hepatitis A-B vaccine, a therapeutic vaccine against hepatitis C, vaccines against cervical cancer, herpes simplex, HIV and tuberculosis.

Before a new vaccine appears on the market, it has to undergo numerous strictly controlled phases. This begins with a non-clinical research phase, followed by a clinical research phase.

The Center for Vaccinology is as equally involved with the non-clinical as it is with the clinical research phase. The clinical studies that we conduct in collaboration with, and on request of, different sponsors are mainly phase I and II studies.

1. Non-clinical research

A vaccine has to stimulate our immune system to produce, amongst others, antibodies which protect the individual against infection with a certain germ or infecting agent (e.g. a virus or bacteria). For this reason, research is first conducted on the immune defence mechanisms of the human body against a certain infecting agent. A candidate vaccine may only be further developed from this stage after approval by the government and medical Ethics Committee.

Scientists then test different preparations on cells, tissues and animal models. These experiments are used to determine whether a vaccine causes any serious side effects. Once the results look promising, the clinical phase begins.

2. Phase I and II clinical studies

At this phase of study, hundreds of healthy volunteers receive the vaccine. Here the emphasis is on safety of the product and possible short term side effects. The effect that vaccination has on the immune system is also evaluated by measuring the amount of antibodies produced in the blood. At this phase of study the ideal dose of vaccine is also determined.

3. Phase III clinical studies

The purpose of this phase of study is to prove that administration of a vaccine is able to fight the virus or bacteria in an efficient and safe manner. Between hundreds and thousands of volunteers participate in this phase of a study.

4. Registration

If a vaccine is found safe and efficient, a licence is requested by an authorized governmental institution. It is only once a vaccine is registered that it can be sold by a pharmacy.

Since 14th November 2005, CEVAC has been based in a new, modern and stylish location on the Ghent University Hospital campus.

Services

CEVAC laboratories is providing central lab services to both industrial and academic research communities. Not limited to, but mainly services are provided in the field of (pre) clinical vaccine evaluation.

Cellular Immune Response measurement is becoming of higher interest now vaccines are designed for diseases where antibody eliciting vaccines are not providing the needed protection (HIV, HCV, Tuberculosis,…).

15 years ago CEVAC laboratories was involved in the design of these initial Cell Mediated Immunity tests. The build-up of expertise through the years have led to a knowledge base providing continuous improvement of these CMI test and a laboratory specially designed, staffed and equipped for performing these Measurements of Antigen Specific Immune Responses at high pace and with excellent quality.

As new compounds are introduced into vaccines no appropriate lab tools are available for quantifying or detecting this vaccine response. Therefore CEVAC designed special lab ‘formats’ that can be rapidly modified in order to obtain a specific lab tool of high quality. The latter reducing the development and validation time of these crucial lab assay and speeds up the whole new drug development process.

Recently highly specialised models have been added to our portfolio permitting the culture of viral organisms (HBV, HCV, HIV,…) in humanised systems. These systems are enormously valuable for the evaluation of antiviral compounds.

All services provided by CEVAC labs are within the scope of a Quality Assessment system that is challenged by both national and international organisations.


Website
www.cevac.be


Laboratory of Immunology and Animal Biotechnology

Research

Chlamydophila psittaci and Chlamydia trachomatis infections

The most important animal chlamydiosis of zoonotic character is psittacosis, a systemic disease in psittacine birds of acute, protracted, chronic or subclinical manifestation. It is a notifiable disease in Belgium, Germany, Ireland, Switzerland, the UK and other countries. The analogous infection in domestic and wild fowl is often called ornithosis.

The economic damage in connection with outbreaks in poultry flocks can be considerable. Although the causative agent, Cp. psittaci, is known to be very wide spread in many avian species, not all carrier birds actually show symptoms of disease. Present knowledge about factors contributing to the development of clinical disease, including virulence factors of field strains, is rather limited. The main purpose of our research is developing new molecular diagnostic methods for identifying and typing the bacterium as well as studying the bacterium host cell interaction in order to develop innovative prophylactic measurements like for instance recombinant protein and DNA vaccination.

At present, we are focusing on type III secretion in Chlamydophila psittaci. We recently identified a type III secretion machinery in this obligate intracellular bacterium and found it to be essential for the bacterial pathogenesis. Additionally, we are focusing on preventing the infection from being spread from birds to man as the agent can cause severe respiratory disease in man.

C. trachomatis infections have an enormous impact on human health. C. trachomatis is the most common cause of sexually transmitted infections throughout the world. Infections are asymptomatic in most females (>75%) and can result in scarring and fibrosis of urogenital tissues, resulting in chronic pelvic pain, ectopic pregnancy, and pelvic inflammatory disease, which can lead to infertility. Acute C. trachomatis infections can be treated effectively with antibiotics, although, once infection and pathology are established, treatment may not prevent complications. Therefore, treating only symptomatic patients will never control the spread of infection.

Asymptomatic individuals can be identified through screening programmes, but this approach is likely to be too costly for developing countries. A vaccination programme would be much cheaper and have a greater impact on controlling C. trachomatis infections world­wide. Computer modeling suggests, that even a partially efficacious chlamydial vaccination programme would rapidly reduce the prevalence of genital infection. In order to develop effective C. trachomatis vaccines, it is important to identify those antigens that elicit a protective immune response and also to develop new and adequate methods and adjuvants for effective vaccine delivery as conventional methods have failed to induce protective immunity.

Trachoma, an ocular infection caused by related C. trachomatis serovars, is the major cause of preventable infectious blindness. Development of a vaccine against urogenital C. trachomatis infections would open the way to a vaccine against trachoma as well. The main objective of our C. trachomatis research is to develop a candidate vaccine against genital Chlamydia (C.) trachomatis infections in females. The vaccine should generate and sustain an adequate protective immune response at the mucosal surface and should also offer protection against all genital serovars of C. trachomatis. Adequacy and efficacy of the candidate vaccine should be measurable by either complete prevention of infection or restriction of infection and prevention of complications.


Website
www.molecularbiotechnology.ugent.be

Laboratory of Biochemistry and Molecular Cytology

Research

Our main topics are telomere biology and the DNA damage response. We investigate the effect of oxidative stress, radiation and physical stress on the single cell level.

Telomeres are sequences that shield the chromosome ends, thereby preventing them from being recognized by the DNA damage response. In normal somatic cells, telomeres shorten with each division until they reach a critical length. Once attained this alerts the cell’s damage checkpoints, signalling it either into senescence or apoptosis.

By scoring the telomeric signals after hybridization with PNA probes and correlating them with telomere lengths (Q-FISH), we are able to map the replicative state of certain cell types, such as human lymphocytes.

Furthermore we are interested in how telomeres are organized and move in the interphase nucleus. Using GFP-coupled telomere binding proteins and confocal time-lapse microscopy we study telomere dynamics in its cellular context. Early results suggest that most telomeres in ‘healthy’ cells are firmly anchored, possibly to a nuclear matrix. Currently we are studying the effect of different kinds of stress factors on telomere distribution and mobility.

DNA damage induces the formation of single stranded (ss) and double stranded (ds) breaks. ³H2AX specifically localizes to ds breaks and recruits DNA damage repair proteins to the ds break site. ³H2AX marks the repair foci very rapidly and dissolves after or during repair. Using very local X-irradiation to induce DNA damage, we study the formation and dynamics of ³H2AX foci in target cells as well as neighbouring cells.

Website
www.molecularbiotechnology.ugent.be

Laboratory of Pharmaceutical Technology

Research

The research is focused on the development of innovative drug dosage forms (mainly solid dosage forms) for human application as well as for veterinary use.

These dosage forms are based on pharmaceutical accepted excipients used as such or as mixtures to impart specific drug release properties (immediate, controlled or sustained release) to the formulation.

The projects at the Department of Pharmaceutical Technology are focused on the following topics:
  • extrusion/spheronisation
  • bioadhesion
  • controlled release based on hot stage extrusion technology
  • freeze-drying
  • tabletting and
  • granulation
These projects involve fundamental research, technical development and in-vitro evaluation of the newly developed formulations as well as a biopharmaceutical part which evaluates the in-vivo efficiency of the formulations following administration to animals or human volunteers.

Next to formulation research several projects involve specific pharmaceutical problems such as the quality of pharmaceuticals in 3rd world countries, the bioavailability of drugs used in palliative care and the development of an alternative method (using naked snails) to determine the mucosal irritation of drugs.

Due to the revolution within the pharmaceutical biotech-industry and to the importance of the parenteral route as the main route of administration of biotech drugs, research work in this field will be initiated at the Department of Pharmaceutical Technology.


Services

Formulation of dosage forms including production at lab-scale.

Characterisation of solid dosage forms via
  • in-vitro dissolution (USP methods 1, 2 and 3)
  • hardness, friability and desintegration time of tablets (Eur. Pharm.)
  • compression properties of drugs and excipients (using an excentric press and compaction simulator)
  • bioadhesion test of tablets

Physical characterisation of materials
  • flow properties of powders and granules (Eur. Pharm.)
  • particle size distribution (sieve analysis and laserdiffraction)
  • thermal analysis (Differential Scanning Calorimetry)
  • water content (IR-oven, Karl Fischer titration)
  • viscosity
  • pH-determination
  • bulk and tapped volume
  • true density (He-pycnometry)
  • pore size distribution (Hg-porosimetry)

Development and optimalisation of freeze-drying cycles


Website
www.ugent.be/fw/farmtech

Laboratory of Pharmaceutical Biotechnology

Research

The research is focused on the following topics :

Optimization of DNA analysis using STR technology

Besides routine DNA typing for the department of justice, our lab performs research in the field of DNA fingerprinting for optimising and improvement of the procedures. We created already our own multiplex of four STR loci and investigated the influence of dactyloscopic powders on DNA analysis.

At this moment we are investigating the use of immunomagnetic beads to purify and isolate specific cell types from mixed forensic samples. This way sperm cells could be isolated before DNA extraction avoiding time-consuming differential DNA extraction. Forensic mixtures in general can be avoided when this mixed sample consists of different cell types.

Rheumatology : A close encounter with Proteomics

Proteomics is defined as the large-scale characterisation of the entire protein complement of a cell line, tissue and organism. It provides us with a more global and integrated view of biology by studying all the proteins of a cell rather than each one individually. With the completion of the sequencing of several genomes it became clear that a lot of information could not be obtained by studying genes alone. It is impossible to elucidate mechanisms of disease, ageing and effects of the environment solely by studying the genome.  Proteomics can provide us with a powerful tool for studying and understanding disease mechanisms and can actively contribute to the search for new drug targets.

Because of their nature of pathology, rheumatoid diseases have and are still extensively studied and looked at from an immunological point of view. However, proteomics is catching up with them! We are currently looking at the proteome of several rheumatoid tissues of different rheumatoid diseases in order to find differences in the expressed protein patterns. This study will provide us a better insight in the pathology of these diseases.

Proteome analysis of mouse embryonic fibroblasts (MEFs) by 2D-PAGE and mass spectrometry

Natural killer T (NK T) cells are a recently discovered line of T lymphocytes. There is increasing evidence of their regulatory function in several immune processes. Their differentiation pathway requires the interaction with the lymphotoxin b receptor (LTbR) expressed on stromal cells. This interaction promotes the stromal cell to produce several growth factors. Proteome analysis of different MEFs (LTbR stimulated versus non-stimulated MEF) reveals which proteins are potential growth factors.

Services

DNA fingerprinting (human)

The Laboratory of Pharmaceutical Biotechnology performs human identification by the use of DNA profiling techniques, mainly for the department of justice in Belgium. The laboratory has a Beltest accreditation since the 15th of September 1996 for DNA isolation and fingerprinting.

Through the combination of the short tandem repeat Loci (STR) in a multiplex polymerase chain reactions (PCR) individuals can be identified with very high levels of certainty. By using PCR technology profiles can be obtained (and thus identification) of very minute and old, badly stored samples (e.g. cigarette butts, blood(stains), saliva(stains), sperm(stains), skin debris, hair, biological tissues, ...):

In our laboratory all 13 STR CODIS loci (loci recommended by the FBI) can be performed (= D3S1358, vWA, FGA, TH01, D21S11, D8S1179, D18S51, TPOX, CSF1PO, D16S539, D13S317, D5S818 and D7S820), including sex determination by determination of the amelogenin locus on the X- and Y-chromosome.

Besides the commercially available kits we also created our own multiplex of 4 STR loci (CD4, TH01, D21S11 and SE33). This multiplex is described and published in 1998 in Electrophoresis. Our multiplex of 4 STR loci shows a great sensitivity and specificity which is much better than the available commercial kit as presented on the Second European Symposium on Human Identification in June 1998 in Innsbruck (DNA typing of fingerprints on skin debris: Sensitivity of Capillary Electrophoresis in forensic applications using multiplex PCR).

This way 15 different STR loci can be determined with sex determination. All STR loci are analysed on a ABI310 and/or ABI3100. The overlapping loci between the available commercial kits and our multiplex (i.e. TH01 and D21S11) give an extra control on the obtained profile.

In special, very urgent cases DNA identification can be performed within 24 hours after receipt of the samples (max. 2-3 samples). Regular cases are performed in max. 3 months.


DNA fingerprinting (animals)

Besides DNA profiling of samples of human origin, this laboratory can also perform DNA typing on cattle and horses. Other animals e.g. dogs under request.


Mitochondrial DNA

The analysis of mtDNA (mitochondrial DNA) is performed by sequencing in order to detect point mutations that are maternally inherited. DNA-sequencing however is a time consuming technique. Mass spectrometry could be very useful for genotyping of forensic samples in gaining important time.

The technique, which is mostly used today, is the determination of oligonuleotides by MALDI-TOF MS (matrix-assisted laserdesorption / ionisation time-of-flight mass spectrometry). The resolution of this technique however is momentarily too small to analyse PCR products that are longer than 100 bases. A Q-TOF MS (quadrupole time-of-flight MS), which combines an electrospray to time-of-flight MS, is installed in our laboratory. This Q-TOF MS will be used for analysing nuclear and mitochondrial DNA.

Ghent Research Group on Nanopharmacy

Research

The Laboratory of General Biochemistry and Physical Pharmacy hosts the Ghent Research Group on Nanopharmacy (GRCN).

Nanopharmacy aims at applying nanotechnology in drug therapy and medical diagnostics. Advanced drug delivery and medical diagnostics constitute the core disciplines of GRCN. Advanced drug delivery systems are nano-/micrometre sized delivery systems for biological therapeutic agents (like nucleic acids and proteins) which are much larger than classical drugs like for example aspirin.

The GRCN is a multidisciplinary research environment, including pharmacists, material engineers, cellular biologists and biophysics who build scientific and technical expertise between "nano" and pharmacy.

Our expertise focuses on the following areas :

Pharmaceutical carriers for gene therapy

With regard to DNA therapy the Laboratory of General Biochemistry and Physical Pharmacy evaluates the use of non viral carriers for DNA delivery. Such 'DNA particles' (which are between 50 and 500 nm in size) consist of DNA and cationic polymers or cationic lipids.

Our DNA delivery research is especially focused on the mobility and the stability of non-viral DNA particles in the extra- and intracellular matrix.

To better understand the cellular behavior of non viral gene DNA complexes, we evaluate the potentials of new biophysical (microscopy) techniques like fluorescence correlation spectroscopy, fluorescence recovery after photobleaching,...

We believe that, in order to obtain real breakthroughs in the design and the understanding of the behavior of the DNA particles in cells, there is an urgent need for advanced biophysical methods which allow to characterize the critical steps in cells that govern gene transfection.

By modern biophysical methods we study e.g. the intracellular release of the DNA from the carriers, the intracellular degradation of DNA, the mobility of naked DNA and DNA particles in the cytosol, the uptake of DNA by the nuclei,...

Pulsed delivery of macromolecules drugs

Pharmaceutical research aims to design drug delivery systems that respond to therapeutic needs. To correlate with our biological needs, precisely timed drug delivery is required. Precisely timed therapy could be improved by the use of 'programmable dosage forms' which deliver the drug at well defined times after e.g. intramuscular or subcutaneous injection.

The Laboratory of General Biochemistry and Physical Pharmacy aims to design 'exploding particles' for pulsed release of macromolecular therapeutics. The pulsed delivery concept we are investigating is based on the use of degradable hydrogels. A degradable gel is encapsulated in the interior of a micron sized capsule along with the drug of interest . At the time the polymer gel becomes a polymer solution the swelling pressure suddenly increases inside the capsule. This results in a rupture of the capsule followed by drug release.

Services

Enzyme analysis

The Laboratory of General Biochemistry and Physical Pharmacy is officially approved to perform analysis of pharmaceutical enzymes on behalf of the Belgian Ministry of Health.

The laboratory is also a member of the European Network of Official Medicines Control Laboratories (OMCL).  It also performs analysis of pharmaceutical enzymes ordered by the pharmaceutical industry.


Contract work

Our contract work especially deals with the physicochemical characterization of solutions and solids like:

  • viscosity and elasticity of materials
  • size characterization of nano- and microparticles
  • zeta-potential measurements
  • molecular weight determination of polymers
  • determination of diffusion coëficient
  • intrinsic viscosity mesaurements
  • osmometry
  • osmolality measurements


Website
www.ugent.be/fw/biofys


Department of Rheumatology

To be updated


Centre for Clinical Immunology and Allergy

To be updated


Laboratory of Bacteriology

The mission of the Laboratory of Veterinary Bacteriology and Mycology is to promote knowledge on veterinary bacteriology and mycology through education, research and public services.

Research

The main research interests of the laboratory are : 


1) host-pathogen interactions of veterinary and / or zoonotically important bacteria and fungi.
2) antimicrobial resistance in veterinary and / or zoonotically important bacteria and fungi.

Expertise

Animal species used for research purposes: Pigs, poultry, pigeons, mice, gerbils, reptiles, amphibians.


The laboratory is approved for research activities with these animal species, meeting both ethical and biosafety requirements to perform infection experiments up to an L2 level, including GMOs. All infection experiments are carried out in A2 stables, adapted to meet all ethical and biosafety requirements.

Microorganisms:
1) Host-pathogen interaction studies: Salmonella (several serovars), Campylobacter jejuni, Helicobacter (several gastric and enterohepatic species), Clostridium perfringens, Batrachochytrium dendrobatidis, Aspergillus fumigatus, Mycoplasma hyopneumoniae, Actinobacillus pleuropneumoniae, Staphylococcus aureus
2) Antimicrobial resistance: bacteria of veterinary and / or zoonotic importance (please inquire for specific taxa)

In vitro models:
1) Adhesion, invasion and cytotoxicity assays using several cell lines and primary cell cultures.
2) Determination of Minimal Inhibitory Concentrations (MIC) for various veterinary and / or zoonotically important bacteria.
3) Virulence gene expression of Salmonella.

In vivo models:
Pigs Salmonella:
Several infection models are used, inoculating 3 to 4 week-old piglets either with a single Salmonella strain or with multiple strains (mixed infection):

  • colonization (up to 5 days post inoculation)
  • persistency (up to 28 days post inoculation)
  • stress induced re-excretion (28 days post inoculation)
  • intestinal loop model (max 12 hours)

 


Poultry Salmonella:

  • day-old to 10 day-old chicks
  • laying hens for egg contamination
  • seeder model in broilers
  • intestinal loop model (max 24 hours for cecal loops)

 


Poultry Campylobacter jejuni:

  • inoculation of 10 to 14 day-old chickens
  • seeder model
  • intestinal loop model

 


Poultry Clostridium perfringens: reproduction of necrotic enteritis lesions
Pigeons Salmonella Typhimurium: reproduction of pigeon paratyphoid
Pigeons Aspergillus fumigatus: reproduction of avian aspergillosis
Gerbils and mice gastric Helicobacter species: reproduction of gastric lesions
Pigs Mycoplasma hyopneumoniae: reproduction of enzootic pneumonia
Reptiles Salmonella: persistent colonization
Pigs Actinobacillus pleuropneumoniae
Rabbits Staphylococcus aureus


Management

Business Developer Dr. ir. Sven Arnouts
Technology Developer Dr. ir. Peter Delputte
Prof. Dr. Eric Cox (promotor)
Prof. Dr. Bruno Goddeeris
Prof. Dr. Dieter Deforce
Prof. Dr. Jo Demeester
Prof. Dr. Stefaan De Smedt
Prof. Dr. Dirk Elewaut
Prof. Dr. Herman Favoreel
Prof. Dr. Freddy Haesebrouck
Prof. Dr. Bart Lambrecht
Prof. Dr. Geert Leroux-Roels
Prof. Dr. Hans Nauwynck
Prof. Dr. Jean Paul Remon
Prof. Dr. Patrick Van Oostveldt
Prof. Dr. Daisy Vanrompay
Prof. Jozef Vercruysse

Towards joint research and knowledge transfer activities

PROVAXS wants to facilitate knowledge transfer activities and research collaboration with other research institutes and industrial companies.

Our Technology Offers describe technologies with clear practical applications that are part of our IP-portfolio.

R&D projects are an invitation to join us in early stage development programs that already showed a proof of concept but need some further exploratory research in order to define clear target applications in the market.

We also offer Tools and Test Models that you can use for screening and testing of the biological activity of your material.

We kindly invite you to examine if our technologies can match your company strategy and market needs and can be converted into useful products or services.


Technology offers

Permissive cells for PRRSV production

• The PRRS-virus can infect the cells via the natural route due to the presence of both sialoadhesin and CD163, the two receptors that are essential for binding, internalization and multiplication

• On average Sn+CD163 cells can produce PRRSV at titers of 1 log higher than in MARC-cells

• Multiple PRRSV-strains can be grown on the Sn+CD163 cells which offers flexibility in production (e.g. for multistrain registration)

• The cells can be used for QC or in a potency test to demonstrate structural and functional integrity of the PRRSV as long as it can bind and internalize

• Since the PRRS-virus can follow its natural route of infection no or little virus adaptation is expected

You can download a Technology Offer here.

Effective and safe inactivated viral vaccines

Our technology enables you to check the antigenicity (efficacy) and safety of your inactivated viral vaccine in vitro

A quick and safe way to develop safe and effective viral vaccines against emerging diseases or new aggressive strains

Fully documented proof-of-concept study on a PRRSV-vaccine, that is capable of inducing neutralizing antibodies upon vaccination and protection upon challenge with live virus

Complete toolkit for the production of an inactivated PRRSV-vaccine including

  • Antibodies to check structural integrity of critical viral proteins
  • Diagnostics to check the antigenicity of the inactivated virus

 Please download the Technology Offer for more information.

PCV2 : virus production

Our invention describes a way to obtain higher titers of Porcine Circovirus 2  in cell cultures for the production of vaccines.

We can increase PCV2 production in cell culture with 2 log10 TCID50/10e5 cells.

Please download the Technology Offer for more information.

PCV2 : identification of different strains

We describe monoclonal antibodies that can be used to identify antigenic differences between PCV2 strains.

Based on this identification the strains can be assigned to clusters with varying virulence.

The technology can thus be used for diagnosis and for selection of strains that should be included in a vaccine to obtain a broad protection against PCV2.

The monoclonal antibodies are described by Lefebvre et al. in Journal of General Virology 2008, 89, 177-187. 

Please download the Technology Offer for more information.

Ostertagia ostertagi antibody ELISA - LICENSED

Ostertagia ostertagi is one of the most important gastrointestinal nematodes for cattle worldwide.  As Ostertagia ostertagi is present on pasture, all grazing cattle in temperate climate regions are exposed to the parasite.


Nematode infestations in adult cows are predominantly subclinical, but may lead to a decrease in milk production.  The screening for Ostertagia ostertagi antibodies in cattle milk samples is a promising tool to follow the infestation level, thus becoming an instrument to determine the need for anthelmintic control.


Our Department of Virology, Parasitology and Immunology-Faculty of Veterinary Medicine has developed an O. ostertagi-Ab ELISA to detect Ostertagia ostertagi specific antibodies in milk.  The test is commercialised by Svanova. (www.svanova.com/inside.asp)

Entry portal through the intestinal barrier

Our technology enables controlled transfer of biological compounds through the intestinal (mucosal) barrier

Via selective transcytosis through the intestinal barrier various functions at the level of the intestinal epithelium and beyond can be modulated

  • antigens can be efficiently presented to the gut associated lymphoid tissue (GALT) leading to a protective mucosal immune response
  • drugs or drug-containing (nano-)particles can be transferred and delivered across the intestinal barrier

Blocking the gateway through the intestinal barrier can reduce the colonisation of and passage through the intestinal epithelium by bacteria, more specifically by E. coli (F4 or K88 positive) , well known for causing diarrhoea in animals and man.

Please download the Technology Offer for more information.

The first vaccine for Psittaciformes against Cp psittaci

We have developed a codon-optimized, MOMP-based DNA vaccine that significantly protects birds (Psittaciformes) against a high challenge with the zoonotic pathogen Chlamydophila psittaci (Cp psittaci).

The vaccine

  • can be used in the presence of circulating antibodies against Cp     psittaci
  • induces a high cellular immune response, which is more important for protection against the intracellular pathogen than high antibody titers
  • can be useful for prevention of the infection (e.g. in breeding facilities or zoos)

The vaccine can significantly reduce the infection of people that are in contact with Psittaciformes like breeders, veterinarians, pet bird owners but also the public at large.

Genotype specific detection of Chlamydophila psittaci

  • The technology comprises the qualitative and quantitative detection of ompA genotypes of Chlamydophila psittaci (zoonotic agent).
  • The method is based on the hybridization of oligonucleotides with the DNA of Chlamydophila psittaci (quantitative PCR) using internal probes and optionally competitor probes which increase specificity.
  • The technology also describes a strain of Chlamydophila psittaci with a novel genotype E/B and methods to distinguish this novel genotype from existing genotypes.

 

Specialized medium (ChlamyTrap) to collect aerosolized obligate intracellular microorganisms

  • The technology comprises a novel medium, which can be used for bioaerosol monitoring of live obligate intracellular pathogens such as viruses and bacteria.
  • The medium is not toxic to eukaryotic artificial hosts for obligate intracellular microorganisms (e.g. cell cultures or embryonated eggs).
  • The medium can also be processed for PCR and does not inhibit polymerase activity.
  • The medium can be used in aerosol impactors combining the high sensitivity and speed delivered by these devices with the possibility of concentrating small microorganisms present in the medium by (ultra)centrifugation prior to cultivation.

 

Powder vaccine for mass vaccination of poultry

We formulated a powder vaccine for aerosol vaccination of poultry.

The vaccine is produced in a one-step spray drying process and is stable during 10 months of storage (tested for a Newcastle disease vaccine).

More information on the powder vaccine is available in the publication that you can download here and in WO 2007/104562.

 

 

Growth factor and biomarkers for chondrocytes and ACT

We have identified a set of 3 biomarkers of phenotypic stability and biological activity of chondrocytes in cell culture. One of the markers can be measured in the culture medium. Moreover, this marker is also a growth factor that stimulates cartilage matrix production by the chondrocytes.


The level of expression of the markers can be used as a quality standard for chondrocyte cultures and to optimize the culture conditions.


The growth factor can control the phenotypic stability or differentiation of chondrocytes and stimulate or maintain the cell’s capacity to produce cartilage matrix.


You can find more information on the markers and the growth factor in the Technology Offer that you can download on this page.

Methods for predicting the efficacy or outcome of HCV therapy

  • We have developed methods for predicting and monitoring the efficacy or outcome of therapies against the Hepatitis C virus (HCV)
  • The method is based on diagnostics comprising the detection of antibodies against NS4/NS5 peptides and diagnostics for the determination of viral titers (HCV RNA or HCV core antigen).
  • The data are combined in a novel algorithm that can predict the outcome of a HCV therapy as early as at 1 week after the start of treatment and with the same sensitivity and specificity as the algorithm that is currently applied (prediction after 12 weeks of treatment).

 


We are happy to collaborate with you on the following R&D projects

Universal applications of DNA vaccines

In a traditional vaccination, protein becomes or live attenuated bacteria or viruses are administered which can cause allergies, fever or other symptoms as side reactions. DNA vaccinatie, vaccinatie with genes, does not have these disadvantages and can lead to the same immune response.

However, while results of DNA vaccination  are very positive in mice, immune responses are still too weak in larger animals and man.  In het PhD work, Dr. Vesna Melkebeek made major steps to increase the immunogenicity of DNA vaccines.  She obtained high antibody titres in pigs after only one vaccination.

We are now looking for partners that want to cooperate to futher develop these research findings to practical applications of DNA vaccination.


Vesna.Melkebeek@UGent.be

Vaccines for Chlamydia trachomatis infections in humans

The main objective of our C. trachomatis research is to develop a candidate vaccine against genital Chlamydia (C.) trachomatis infections in females. The vaccine should generate and sustain an adequate protective immune response at the mucosal surface and should also offer protection against all genital serovars of C. trachomatis. Adequacy and efficacy of the candidate vaccine should be measurable by either complete prevention of infection or restriction of infection and prevention of complications.

Vaccines for Chlamydophila psittaci in poultry and pet birds

The main purpose of our research is developing new molecular diagnostic methods for identifying and typing the bacterium as well as studying the bacterium host cell interaction in order to develop innovative prophylactic measurements like for instance recombinant protein and DNA vaccination. Antigenic proteins and their B and T cell epitopes have already been identified. Vaccines are being evaluated in experimental infection models using both turkeys and budgerigars.

New strategies to reduce O157:H7 E. coli (EHEC) infections

The first goal of this project is to develop a fast and sensitive immunological screening and detection test by studying the immune response against different virulence factors of E. coli O157:H7. Since some toxins of EHEC are capable of suppressing lymphocytes, the study of systemic and mucosal immune responses may provide information about the rise of persistently infected animals.

The second goal is the study of systemic and mucosal immune responses.

The third goal is to inhibit excretion/contamination. In this aspect, the development of a vaccine is a long-term strategy. The effect of mucosal administration of lactoferrin on colonisation and excretion will also be investigated.

Adjuvants for mucosal immune responses

Vaccines that can be delivered via the mucosal route could boost the control of many infectious diseases both in human and in animals.  The most practical application would be via the oral route.

However, the number of available mucosal vaccines is very limited which is due to important difficulties as compared to systemic vaccination.  Some of these difficulties can maybe be overcome by the use of mucosal adjuvants.  You can find an overview of candidate adjuvants in the document that you can download here.

We offer in vivo and in vitro models in which we can evaluate the effect of candidate adjuvants on the generation of a mucosal immune response.


News

Modern Mucosal Vaccines, Adjuvants & Microbicides

28-04-2010 - Author: Sven Arnouts"

PROVAXS will partcitipate at the conference Modern Mucosal Vaccines, Adjuvants & Microbicides with the following contributions (oral
presentation) :


'Prophylactic use of transferrins against chlamydiosis.


Daisy Vanrompay, Delphine S.A. Beeckman and Caroline Van Droogenbroeck (Ghent University, Ghent, Belgium)


'The food contaminant fumonisin B1 reduces the maturation odf porcine CD11R1+intestinal antigen presenting cells and antigen-specific immune respnses, leading to a prolonged intestinal ETEC infection.'' 

Bart Devriendt, Melanie Gallois, Frank Verdonck, Yann Wache, Diane Bimczok, Isabelle P. Oswald, Bruno M. Goddeeris and Eric Cox (University of Ghent, Ghent, Belgium)

'Maltose-binding protein (MPD) is a potential carrier for oral immunizations'.

Philippe Bellot, Petra Tiels, Frank Verdonck, Vesna Melkebeek, Bruno M. Goddeeris and Eric Cox (University of Ghent, Ghent, Belgium)


You can download a brochure of the conference in attachment

 



 

 


Vaccine Innovation

27-04-2010 - Author: Sven Arnouts"



Sven Arnouts (Business Developer PROVAXS) will participate at 

 



from April 27-29, 2010 in Frankfurt, Germany


If you would like to meet him there please contact him at

sven.arnouts@ugent.be or +32 495 707 334

Vaccine Innovation


Respiratory coronavirus and bacterial cell wall toxins

12-02-2010 - Author: Sven Arnouts"

On February 12, 2010 Kalina Atanasova will defend her PhD thesis on

Interactions between porcine respiratory coronavirus and bacterial cell wall toxins in the lungs of pigs

You are kindly invited to attend the defence at the Faculty of Veterinary Sciences, Salisburylaan 133, 9820 Merelbeke (start 16.00pm, auditorium Hoogbouw)

You can download an invitation to the PhD defence


Treatment of bacterial respiratory disease

11-02-2010 - Author: Sven Arnouts"

On February 11, 2010 An Garmyn will defend her PhD thesis on

Challenging the prescribed enrofloxacin treatment regimen of bacterial respiratory disease caused by Ornithobacterium rhinotracheale and Escherichia coli in turkeys

You are kindly invited to attend the defence at the Faculty of Veterinary Sciences, Salisburylaan 133, 9820 Merelbeke (start 17.00pm, auditorium D)


You can download an invitation to the PhD defence (Dutch)


Wetenschapscafé 'Virussen en vaccins'

09-02-2010 - Author: Sven Arnouts"

Hierbij nodigen wij u van harte uit op het

Wetenschapscafé op dinsdag 9 februari 2010 van 19.45u tot 21.45u met als thema :

Virussen en vaccins

Sprekers : prof. Geert Leroux-Roes, prof. Hans Nauwynck

Moderator : Joël De Ceulaer

Lokatie : Zebrastraat NV, Zebrastraat 32, 9000 Gent (in buurt van Decascoop)

www.wetenschapscafegent.be/cafe/1265760000

www.wetenschapscafegent.be/


Lab for Parasitology recognized as WHO-partner

22-01-2010 - Author: Sven Arnouts"

The Lab for Parasitology of the Faculty of Veterinary Medicine has officially been recognized by the WHO (World Health Organization) as the 'Collaboration Centre for the monitoring of anthelminitic drug efficacy for soiltransmitted helminths'.

The lab is therefore responsible for the follow-up of the research on the efficacy of anti-helminthics for humans. Worm infections are the most occurring disease for humans and animals.

Worldwilde, about 1,2 billion of people are contaminated. The disease occurs mostly in the tropics.

Professor Jozef Vercruysse, Lab for Parasitology, Department Virology, Parasitology and Immunology, +32 9 264 73 90, Jozef.Vercruysse@UGent.be


Interspecies transmission of the influenza viruses

16-12-2009 - Author: Sven Arnouts"

On December 16, 2009 Annebel De Vleeschauwer will defend her PhD thesis on

Pigs and avian influenza viruses: susceptibility and significance for interspecies transmission

You are kindly invited to attend the defence at the Faculty of Veterinary Sciences, Salisburylaan 133, 9820 Merelbeke (start 16.00pm, auditorium D)

You can download an invitation to the PhD defence (Dutch)


Assembly and Transport of Neuroinvasive Herpesviruses

01-12-2009 - Author: herman.favoreel@ugent.be"

On Tuesday, December 1st, at 3pm, Dr Greg Smith from Northwestern University in Chicago will give aseminar on

'Assembly and Transport of Neuroinvasive Herpesviruses'

in Auditorium D, Faculty of Veterinary Medicine ,Ghent University


You are all very welcome to attend this seminar.


Viral safety for veterinary vaccines

25-10-2009 - Author: Sven Arnouts"

From 25 to 27 Octobre 2009 IABS, in collaboration with IFAH, organises a workshop on

 

Viral safety and extraneous agents testing for veterirary vaccines

 

in Annecy, France.

You can find more information and register on www.viral-safety-2009.com.


Novel insights in the sero-diagnosis and pathogenesis of...

21-10-2009 - Author: Filip Barbé"

On October 21, 2009 Filip Barbé will defend his PhD thesis on :

 

Novel insights in the sero-diagnosis and pathogenesis of swine influenza

You are kindly invited to attend the defence at the Faculty of Veterinary Sciences, Salisburylaan 133, 9820 Merelbeke (start at 4.30 pm, auditorium Hoogbouw)

You can download an invitation to the PhD defense (Dutch).


Entry of FIP in monocytes

17-09-2009 - Author: Evelien Van Hamme"

On 17 September 2009 Evelien Van Hamme will defend her PhD thesis on :

 

Entry of the feline infectious peritonitis virus in blood monocytes


You are kindly invited to attend the defence at the Faculty of Veterinary Sciences, Salisburylaan 133, 9820 Merelbeke (start at 4:30pm ; aud Hoogbouw).


You can download an invitation to the PhD defense (Dutch).

 


Clostridium perfringens and necrotic enteritis

16-09-2009 - Author: Leen Timbermont"

On 16 September 2009 Leen Timbermont will defend her PhD thesis on :

Virulence mechanisms of Clostridium perfringens in broiler necrotic enteritis


You are kindly invited to attend the defence at the Faculty of Veterinary Sciences, Salisburylaan 133, 9820 Merelbeke (start at 5pm ; aud D).


You can download an invitation to the PhD defense (Dutch).


Vaccines for Enteric Diseases

11-09-2009 - Author: Vesna Melkebeek"

Vesna Melkebeek will give a lecture on

 

The use of Gantrez particles for oral delivery of a vaccine to protect pigs against post weaning diarrhoea caused by F4+ enterotoxigenic Escherichia coli

 

at the conference Vaccines for Enteric Diseases, 9-11 September, Malaga Spain.

You can download the programme of the conference from this page.


Influenza in pigs

26-06-2009 - Author: Constantinos Kyriakis"

On 26 June 2009 Constantinos Kyriakis will defend his PhD thesis on :

Surveillance and control of influenza in pigs


You are kindly invited to attend the defence at the Faculty of Veterinary Sciences, Salisburylaan 133, 9820 Merelbeke (start at 4pm ; Auditorium D).


You can download an invitation to the PhD defense (Dutch).


Vaccine Forum & Active Immunotherapeutics

22-06-2009 - Author: Sven Arnouts"

Sven Arnouts (Business Developer PROVAXS) and Peter Delputte (Technology Developer PROVAXS) will participate at

Vaccine Forum & Active Immunotherapeutics

from 22 to 24 June 2009 in Barcelona Spain.

I you would like to meet us there please contact us at :

Sven.Arnouts@UGent.be or +32 495 707 334

Peter.Delputte@UGent.be or +32 477 420 383

 

You can find more information on the conference at www.phacilitate.co.uk/barcelona or download the brochure of the event here.


Recombinant antibodies

16-06-2009 - Author: Peter Delputte"

Peter Delputte will attend the 8th International Congress on

Recombinant Antibodies

from 16 to 18 June 2009 in Cologne, Germany.

You can download the program of the congress on this page.


Production of viral antigens in plants

15-06-2009 - Author: Sven Arnouts"

Annelies De Paepe will present a poster on

Plant seeds as bioreactors for the production of high-value proteins.

at the third international conference on Plant-bades vaccines and antibodies in Verona, Italy (15-17 June 2009).

She will present a technology to produce recombinant proteins in Arabidopsis seeds that can also be used for the production of viral antigens.

For more information you can go to www.meetingmanagement.com/pbva_2009 or download the brochure of the event here.


Ivermectin resistance in Ostertagia ostertagi

11-06-2009 - Author: Annelies Van Zeveren"

On 11 June 2009 Annelies Van Zeveren will defend her PhD thesis on :

Ivermectin resistance in the bovine nematode Ostertagia ostertagi


You are kindly invited to attend the defence at the Faculty of Veterinary Sciences, Salisburylaan 133, 9820 Merelbeke (start at 4pm ; aud Hoogbouw).


You can download an invitation to the PhD defense (Dutch).


NKT cells and arthritis

09-06-2009 - Author: Prof. Dirk Elewaut"

Professor Dirk Elewaut will give a lecture on

NKT cells and arthritis

at the Scientific day 2009 of Miltenyi Biotec on Cross talk between innate and adaptive cells in Brussels (9 June 2006).

You can find more information on the event in the program that you can download here.


Bridging innate and adaptive immunity

28-05-2009 - Author: Bart Lambrecht and Dirk Elewaut"

Professor Bart Lambrecht and Professor Dirk Elewaut together with Abcam organize the

Allergy & Asthma Symposium : Bridging Innate and Adaptive Immunity

May 28-29, 2009 Bruges, Belgium

You can download a draft program here and find more information on

www.abcam.com/conferencecalendar


Immuno-evasion in FIP

27-05-2009 - Author: Els Cornelissen"

On 27 May 2009 Els Cornelissen will defend her PhD thesis on :

Immune evasion of feline
infectious peritonitis virusinfected
monocytes


You are kindly invited to attend the defence at the Faculty of Veterinary Sciences, Salisburylaan 133, 9820 Merelbeke (start at 4:30pm ; aud Hoogbouw).


You can download an invitation to the PhD defense (Dutch).


The role of type III secretion in pathogenesis of Cp psittaci

15-05-2009 - Author: Delpine Beeckman"

On 15 May 2009 Delphine Beeckman will defend her PhD thesis on :

 

Examining the role of type III secretion in the biology and intracellular pathogenesis of Chlamydia psittaci


You are kindly invited to attend the defence at the Faculty of Bioscience Engineering, Coupure Links 653, 9000 Ghent (start at 4pm ; room A0.030).


You can download an invitation to the PhD defense (Dutch).


Doctorate Honoris Causa of Peter Doherty

19-03-2009 - Author: Eric Cox"

As part of the programme to celebrate its 75th anniversary, the Faculty of Veterinary Medicine of the University of Ghent honours Dr. Peter Doherty with a doctorate Honoris Causa on 20 March 2009.

This doctorate Honoris Causa is preceeded by a scientific symposium on T-cell regulation.

You can find the programme of the symposium and more information on www.ugent.be/di/nl/agenda/symposium.htm


Creativity in technology transfer at UGent

17-03-2009 - Author: Sven Arnouts"

We kindly invite you to "Creatief valoriseren" on 17 March.  During this seminar UGent will present her new assets to make our university a cradle for new start-up companies, to stimulate entrepreneurship and to still improve the relations with our industrial partners.

Concrete examples will show how interactions and collaborations between university and industry can be supported by IOF, UGent and GentBC.

The way how PROVAXS is working in this new environment will also be presented.

Please link to  www.techtransfer.ugent.be for more information and to subscribe for this event.


Role of cholesterol in pseudorabies infection

10-03-2009 - Author: Ann Desplanques"

On 10 March 2009 Ann Desplanques will defend her PhD thesis on :

Role of cholesterol and lipid rafts during pseudorabies virus infection

 

See also : www.ugent.be/di/nl/onderzoek/verdedigingen

You can download an invitation to the PhD defense (Dutch).


Job opportunity - Postdoctoral Researcher

10-03-2009 - Author: Peter Delputte"

The Laboratory of Virology is looking for a Postdoctoral Researcher to support their research on new applications of macrophage targeting in the framework of an IWT SBO-project.

You can find more information in the document that you can download here or by contacting Dr. Peter Delputte (Peter.Geldhof@UGent.be).


Diagnosis of porcine cysticercosis

12-02-2009 - Author: Nynke Deckers"

On 12 February 2009 Nynke Deckers will defend her PhD- thesis on

 

Serological markers for improved diagnosis of porcine cysticercosis


See also : www.ugent.be/di/nl/onderzoek/verdedigingen

You can download an invitation to the PhD defense (Dutch).


New possibilities for collaboration with Flemish universities

20-01-2009 - Author: Sven Arnouts"

Two new programs are open to stimulate collaboration between Flemish universities and companies :

  • Baekeland mandates : offering new chances to make a PhD at a university in close collaboration with a company that is responsible for the strategic positioning of the topic and research focus
  • Technology-exploration : offering SME's flexible access to know how and expertise of the university

PROVAXS is open for collaboration with industrial partners in each program and to contribute in this way to innovation and the implementation of new technologies in our field of expertise and know how.

You can find more information in the document that you can download here or you can contact Sven Arnouts at Sven.Arnouts@UGent.be or +32 495 707 334.


Salmonella enteritidis virulence factors

20-01-2009 - Author: Inne Gantois"

On 20 January 2009 Inne Gantois will defend her PhD- thesis on

 

Genome-wide search of Salmonella Enteritidis virulence factors involved in egg contamination


See also : www.ugent.be/di/nl/onderzoek/verdedigingen

You can download an invitation to the PhD defense (Dutch).

 


Diagnostics based on digitally encoded microcarriers

13-01-2009 - Author: Stefaan Derveaux"

On 13 January 2009 Stefaan Derveaux will defend his PhD thesis on :

 

Development of a sensitive diagnostic multiplex platform based on digitally encoded microcarriers

 

You are kindly invited to attend the public defense that will start at 17:30h in "Het Pand", Zaal Rector Blanquaert, Onderbergen 1, 9000 Gent.


PROVAXS certified as Service Provider for innovation

09-01-2009 - Author: Sven Arnouts"

PROVAXS is certified as Service Provider or Center of Expertise for the SME- portfolio of the Flemish Government.  In this position PROVAXS can assist SME's in Flanders in feasability or pilot studies to explore new technonogies or to start a proces of innovation.

With the SME -portfolio SME's can get upto 15.000€ funding anualy for education, exploration of technology or innovation.

You can find more information on www.kmo-portefeuille.be.

The certificate can be downloaded from this page.

 


PCV2, features of different strains

08-01-2009 - Author: David Lefebvre"

On 8 January 2009 David Lefebvre will defend his PhD- thesis on

Genetic, antigenic and pathogenetic features of porcine circovirus type 2 strains


See also : www.ugent.be/di/nl/onderzoek/verdedigingen

You can download an invitation to the PhD defense (Dutch).


Pseudorabies virus in pigs

06-01-2009 - Author: Sarah Glorieux"

On 6 January 2009 Sarah Glorieux will defend her PhD- thesis on

Invasion of pseudorabies virus in porcine nasal respiratory mucosa explants

 

See also : www.ugent.be/di/nl/onderzoek/verdedigingen

You can download an invitation to the PhD defense (Dutch).


C psittaci, epidemiology and vaccine development

19-12-2008 - Author: Kristel Verminnen"

On 19 December 2008 Kristel Verminnen will defend her PhD- thesis on

Epidemiological research on Chlamydophila psittaci zoonosis and development of DNA formulations for mucosal vaccination of turkeys



You can download an invitation to the PhD defense (Dutch).


Nanostructured materials for tissue engineering

12-12-2008 - Author: Stefaan De Smedt"

Dr. Nadia Jessel will give a presentation on

Nanostructured Active Materials for Tissue Engineering applications



December 12th, 2008 at 11h00 in Seminar Room 4 at the Faculty of Pharmaceutical sciences, Harelbekestraat 72, 9000 Ghent, Belgium.

More information is available in the invitation that you can download here.


Photophysically encoded microcarriers

11-12-2008 - Author: Farzaneh Fayazpour"

On 11 December 2008 Mss. Farzaneh Fayazpour will defend her PhD on

Exploring new applications for photophysically encoded microcarriers

You are kindly invited to attend the defence that will start at 4pm in Aud I of the Faculty of Pharmaceutical Sciences.


DNA vaccination and priming of mucosal immunity

07-12-2008 - Author: Vesna Melkebeek"

Dr. Vesna Melkebeek (Laboratory of Immunology) will present the poster entitled


Priming of mucosal response following parenteral DNA vaccination in pigs?


at the 2nd Vaccine Global Congress in Boston, USA from 7 to 9 December 2009.

www.vaccinecongress.com


Targeting dendritic cells

05-12-2008 - Author: Bart Lambrecht"

On Friday 5 December 2008 Professor Bart Lambrecht will give a lecture on

Targeting dendritic cells in inflammatory diseases

during the FWO-minisymposium on New targets in inflammatory and autoimmune diseases (Leuven, Belgium).

You can download the program of the minisymposium here.


PROVAXS participates at Vaccines Europe

02-12-2008 - Author: Sven Arnouts"

PROVAXS will be present at Vaccines Europe, three parallel conferences on

Vaccine Discovery and Development

Vaccine Characterisation and Quality Control

Vaccine Scale-Up and Manufacturing


Several posters are submitted that illustrate recent advances in our technologies.

Vaccines Europe, 2-3 December 2008, Brussels Belgium

www.informa-ls.com/vaccines


Influenza receptors

01-12-2008 - Author: Kristien Van Reeth"

On 1 December 2008, Professor Dr. John Nicholls of the Department of Pathology, University of  Hong Kong will give a lecture on

Influenza receptor and seeds of confusion

Since the outbreak of H5N1 John Nicholls studies the pathology of infections of avian influenza in man.  Currently, his research focuses on influenza receptors in the human respiratory tract that determine the sensitivity to avian influenzaviruses.

Ghent University, Faculty of Veterinary Medicine, auditorium D, 11:30am.


Alphaherpesviruses and the immune system

28-11-2008 - Author: Herman Favoreel"

Professor Herman Favoreel will give an oral presentation on


Alphaherpesviruses and the immune system : a delicate balance


at the Annual Meeting of the Belgian Immunological Society on Friday 28 November 2008.

www.bims.be/anmeet.htm


Protective antigens for vaccinaton against Ostertagia

27-11-2008 - Author: Yves Meyvis"

On 27 November 2008 Yves Meyvis will defend his PhD- thesis on

Isolation and characterization of antigens present in the protective ES-thiol fraction of Ostertagia ostertagi and their evaluation as vaccine candidate

See also : www.ugent.be/di/nl/onderzoek/verdedigingen

You can download an invitation to the PhD defense (Dutch).


Immuno-evasion in PRRSV

25-11-2008 - Author: Sarah Costers"

On 25 November 2008 Sarah Costers will defend her PhD- thesis on

Strategies exhibited by PRRSV allowing evasion of immune detection and/or clearance in the infected pig

See also : www.ugent.be/di/nl/onderzoek/verdedigingen

You can download an invitation to the PhD defense (Dutch).


Job opportunity - Postdoctoral scientist

20-11-2008 - Author: Peter Geldhof"

The Laboratory for Parasitology is looking for a Postdoctoral Scientist to support their research on the role of the gastrointestinal mucus layer in the infection process of gastrointestinal pathogens.

You can find more information in the document that you can download here or by contacting Dr. Peter Geldhof (Peter.Geldhof@UGent.be).


Carbohydrate recognition by Helicobacter pylori

15-10-2008 - Author: Professor Cox"

On 15 Octobre 2008, starting at 11am, Professor Susann Teneberg will give a lecture on

"The complex carbohydrate recognition by Helicobacter pylori"

 

University of Ghent, Faculty of Veterinary Medicine, Auditorium D


Identification of a novel F18 receptor

15-10-2008 - Author: Annelies Coddens"

On 15 October 2008 Annelies Coddens will defend her PhD- thesis on

Expression and Molecular Characterization of the Receptor for F18+ Escherichia coli in Pigs

See also : www.techtransfer.ugent.be/detail_news.asp

You can download an invitation to the PhD defense (Dutch).


Probing the mechanisms of Amyotrophic Lateral Sclerosis

10-10-2008 - Author: Professor Cox"

We kindly invite you to the presentation of Hugo J. Bellen on "Probing the mechanisms of Amyotrophic Lateral Sclerosis".

Hugo J Bellen, DVM, PhD
Investigator, Howard Hughes Medical Institute
Professor, Baylor College of Medicine
Houston, TX, USA

The ALS story is a beautiful example of how basic science mixes with clinical issues with a high impact.

University of Ghent, Faculty of Veterinary Sciences, auditorium B.  You can confirm your participation on http://www.doodle.ch/grar5esftg73w3fk.


PROVAXS participates at EuroBio 2008

08-10-2008 - Author: Sven Arnouts"

Sven Arnouts will participate at EuroBio 2008 on 8 Octobre.  We also participate in the partnering program.

www.eurobio2008.com


Treatment of equine herpes virus 1 with acyclovir

03-10-2008 - Author: Barbara Garré"

On 3 October 2008 Barbara Garré will defend her PhD thesis on

Pharmacokinetics and clinical efficacy of acyclovir in the treatment of equine herpes virus type 1 infections

You can download an invitation (Dutch) for the defense.


New breakthrough towards antibiotic-free pig production

30-09-2008 - Author: Sven Arnouts"

The identification of the receptors of F4 and F18 E coli opens new ways for (preventive) treatment of post-weaning diarrhea (PWD) and edema disease (ED).

PWD is a multifactorial disease caused by both F4+ and F18+ enterotoxigenic E. coli and affects piglets in the first two weeks after weaning. ED is caused by infection with F18+ verotoxigenic E. coli. Antibiotics are extensively used to treat and prevent these diseases, even though this can lead to multiple antibiotic resistant strains and to the spread of antibiotic residues in the environment.

At the Laboratory of Immunology of the Faculty of Veterinary Medicine (member of PROVAXS) we therefore focus on other prophylactic measures against PWD and ED. We already demonstrated that oral vaccination of piglets with F4 fimbriae protects them against an F4+ enterotoxigenic E. coli infection. Our recent discovery of a new F4 receptor (patent pending) opens new ways to improve the efficiency of oral vaccines, not only against F4 but also against other pathogens by targeting this F4 receptor.

Oral vaccination with F18 fimbriae does not result in protection. Therefore we had to develop alternative strategies to control F18+ E. coli. Annelies Coddens (Laboratory of Immunology at the Faculty of Veterinary Medicine) in cooperation with Susann Teneberg (Department of Medical Biochemistry and Cell Biology Institute of Biomedicine at University of Gotheborg, Sweden) identified the F18 receptor (patent pending). This finding opens new opportunities for the development of antibiotic-free treatments that interfere with the colonisation of the pig intestine by F18+ E. coli. Annelies will defend her PhD thesis on the F18 receptor on 15 October at the Faculty of Veterinary Medicine.

For more information you can contact Professor Eric Cox (Eric.Cox@UGent.be ; +32 9 264 73 96) or Dr. Sven Arnouts, Business Developer of PROVAXS (Sven.Arnouts@UGent.be ; +32 495 707 334).


ASP-proteins of Ostertagia ostertagi

25-09-2008 - Author: Aline Visser"

On 25 September 2008 Aline Visser defended her PhD thesis on

Characterisation of activationassociated secreted proteins (ASP) in the bovine abomasal nematode Ostertagia ostertagi

You can download a summary of the thesis (Dutch).


Molecular epidemiology of Cp psittaci in birds and humans

19-06-2008 - Author: Taher Harkinezhad"

On 20 June 2008 Taher Harkinezhad defended his PhD thesis on

Molecular epidemiology of Chlamydophila psittaci in psittacine birds and humans and prevention by DNA vaccination

 You can download 2 publications related to this thesis.


Excretory-secretory products of Ostertagia ostertagi

05-06-2008 - Author: Heidi Saverwijns"

On 5 June 2008 Heidi Saverwijns defended her PhD thesis on

Study of Ostertagia ostertagi excretory-secretory products

You can download the thesis here.


Immuno-evasion in FIP

02-06-2008 - Author: Hannah Dewerchin"

On 2 June 2008 Hannah Dewerchin defended her PhD thesis on

Characterisation of potential immuno-evasion mechanisms of the feline infectious peritonitis virus

 


Identification of a novel F4 receptor

25-04-2008 - Author: Kristien Rasschaert"

On 25 April 2008 Kristien Rasschaert defended her PhD thesis on

Identification of a novel F4 receptor involved in endocytosis and transcytosis.


Lab in the picture : PROVAXS

22-04-2008 - Author: Els Vermeulen"

The Tech Transfer Newsletter GazeTTe! April 2008 includes an article on the genesis of the PROVAXS consortium.  You can download this article here.


Contact

Dr. ir. Sven Arnouts
Business Developer
Center for Strategic Prophylaxis and Vaccine Development - PROVAXS
Ghent University
Faculty of Veterinary Medicine
Department of Virology, Parasitology and Immunology
Salisburylaan 133, 9820 Merelbeke, Belgium
Tel. : +32 495.707.334
Fax : +32 9 264.74.96

Sven.Arnouts@UGent.be